The enzyme methylates 23S rRNA in vitro, assembled 50S subunits are not a substrate [1]. The enzyme specifically methylates guanine1835 at N2 in 23S rRNA.
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S-adenosyl-L-methionine + guanine(1835) in 23S rRNA = S-adenosyl-L- homocysteine + N(2)-methylguanine(1835) in 23S rRNA.