The enzyme specifically methylates guanine2069 at position N7 in 23S rRNA. In ¦Ã-proteobacteria the enzyme also catalyses EC 2.1.1.173, 23S rRNA (guanine2445-N2)-methyltransferase, while in ¦Â-proteobacteria the activities are carried out by separate proteins [1]. The enzyme from the ¦Ã-proteobacterium Escherichia coli has RNA unwinding activity as well [1].
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S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L- homocysteine + N(7)-methylguanine(2069) in 23S rRNA.