The enzyme, characterized from actinobacterium?Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol and cholate. The enzyme is very specific for its substrate, and requires that the side chain at C17 is completely removed.
Cofactor
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Atp + 3-((3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4- yl)propanoate + CoA = Amp + diphosphate + 3-((3aS,4S,7aS)-7a-methyl-1,5- dioxo-octahydro-1H-inden-4-yl)propanoyl-CoA.