| Synonyms |
More, p-cresol methylhydroxylase, p-cresol methylhydroxylase A, p-cresol methylhydroxylase B, p-cresol methylhydroxylases, p-cresol-(acceptor) oxidoreductase (hydroxylating), p-cresolmethylhydroxylase, para-cresol methylhydroxylase, pchC, pchF, PCMH |
| Comments |
This bacterial enzyme contains a flavin (FAD) subunit and a cytochrome c subunit. The flavin subunit abstracts two hydrogen atoms from the substrate, forming a quinone methide intermediate, then hydrates the latter at the benzylic carbon with a hydroxyl group derived from water. The protons are lost to the bulk solvent, while the electrons are passed to the heme on the cytochrome subunit, and from there to azurin, a small copper-binding protein that is co-localized with the enzyme in the periplasm. The first hydroxylation forms 4-hydroxybenzyl alcohol; a second hydroxylation converts this into 4-hydroxybenzaldehyde. Formerly EC 1.17.99.1. |
