Approximately 52.7 kDa, a single non-glycosylated polypeptide chain containing 503 amino acids, with Leu, Glu and 6 × His at C-terminus.
Biological Activity
No data available
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rFlagellin, His as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Fedorov OVandKostyukova AS. 1984. FEBS Lett, 171: 145-8.2. Belas RandFlaherty D. 1994. Gene, 148: 33-41.3. Givaudan A, Lanois A, Boemare N. 1996. Gene, 183: 243-53.4. Meindl T, Boller T, Felix G. 2000. Plant Cell, 12: 1783-94.5. Hayashi F, Smith KD, Ozinsky A, et al. 2001. Nature, 410: 1099-103.
