Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Appearance
Sterile Colorless liquid.
Formulation
A 0.2 um filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0, 30 % glycerol.
Endotoxin
Less than 1 EU/ug of rRtIL-2, Liquid as determined by LAL method.
Reconstitution
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
References
1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.
