| Synonyms |
Bio2, BIO2 protein (Arabidopsis thaliana clone pMP101 gene BIO2 reduced), BioB, biotin synthase, biotin synthase (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2), biotin synthase (Treponema pallidum gene TP0228), biotin synthetase, biotin synthetase (Aquifex aeolicus gene bioB), biotin synthetase (Arabidopsis thaliana clone pMB101 gene BIO2 reduced), biotin synthetase (Bacillus subtilis gene bioB), biotin synthetase (Chlamydia trachomatis gene birA), GenBank AE000716-derived protein GI 2983482, GenBank AE001204-derived protein GI 3322497, GenBank AE001343-derived protein GI 3329182, GenBank AF008220-derived protein GI 2293187, GenBank U24147-derived protein, GenBank U31806-derived protein GI 1403662, GenBank U51869-derived protein GI 1277029, GenBank Z99119-derived protein GI 2635504, NCgl0071, synthetase, biotin, synthetase, biotin (Arabidopsis thaliana clone lambdaBIO2 gene bioB), synthetase, biotin (Arabidopsis thaliana clone pMP101 gene BIO2 reduced), synthetase, biotin (Arabidopsis thaliana clone pYESCBS1 gene bioB), synthetase, biotin (Bacillus subtilis clone pBIO100/pBIO350/pBIO201 gene bioB), synthetase, biotin (Bacillus subtilis gene bioB), synthetase, biotin (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2 reduced) |
| Comments |
The enzyme binds a [4Fe-4S] and a [2Fe-2S] cluster. In every reaction cycle, the enzyme consumes two molecules of AdoMet, each producing 5′-deoxyadenosine and a putative dethiobiotinyl carbon radical. Reaction with another equivalent of AdoMet results in abstraction of the C6 methylene?pro-S?hydrogen atom from 9-mercaptodethiobiotin, and the resulting carbon radical is quenched via formation of an intramolecular C-S bond, thus closing the biotin thiophane ring. The sulfur donor is believed to be the [2Fe-2S] cluster, which is sacrificed in the process, so that?in vitro?the reaction is a single turnover.?In vivo, the [2Fe-2S] cluster can be reassembled by the Isc or Suf iron-sulfur cluster assembly systems, to allow further catalysis. |
