Biotinylated Human IL-18 Protein (Primary Amine Labeling), Accession#: Q14116, CAS#: 3202-706-1720; ChemWhat Code: 1414216
Overview
| Product Description | Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.It contains Tyr37-Asp193. |
| Biotinylated | Yes |
Specification
| Target | IL-18 |
| Synonyms | IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1 |
| Gene/Protein ID (Accession) | Q14116 |
| Species | Human |
| Exact Sequence | Tyr37-Asp193 |
| Molecular Weight | The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-18BP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 26.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | No Tag |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414216 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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