camalexin synthase EC#: 1.14.19.52; ChemWhat Code: 1375817

Product Name camalexin synthase
Example Structure Example Structure of camalexin synthase EC#: 1.14.19.52
Synonyms bifunctional dihydrocamalexate synthase/camalexin synthase, CYP71B15, CYP71B15/PAD3, PAD3
EC Number 1.14.19.52
CAS Registry Number
Comments This P450 (heme thiolate) enzyme, which has been characterized from the plant Arabidopsis thaliana, catalyses the last two steps in the biosynthesis of camalexin, the main phytoalexin in that plant. The enzyme catalyses two successive oxidation events. During the first of oxidation the enzyme introduces a C-N double bond, liberating hydrogen cyanide, and during the second oxidation it catalyses a decarboxylation.
Cofactor heme-thiolate
History
Reactions 2-(L-cystein-S-yl)-2-(1H-indol-3-yl)acetonitrile + 2 [reduced NADPH- -hemoprotein reductase] + 2 O(2) = camalexin + hydrogen cyanide + CO(2) + 2 [oxidized NADPH–hemoprotein reductase] + 4 H(2)O.

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