BAL, bile acid coenzyme A ligase, Bile acid coenzyme A synthetase, Bile acid-coenzyme A ligase, Bile acid:CoA ligase, CCL, Cholate thiokinase, Cholic acid:CoA ligase, Cholic thiokinase, Choloyl coenzyme A synthetase, Choloyl-CoA synthetase, Synthetase, choloyl coenzyme A
EC Number
6.2.1.7
CAS Registry Number
9027-90-1
Comments
Requires Mg2+?for activity. This membrane-bound enzyme catalyses the first step in the conjugation of bile acids with amino acids, converting bile acids into their acyl-CoA thioesters. The second step involves EC?2.3.1.65, bile acid-CoA:amino acid?N-acyltransferase and converts the acyl-CoA thioester into the corresponding?N-acyl amidate by conjugation with glycine or taurine [5]. Chenodeoxycholate, deoxycholate, lithocholate and trihydroxycoprostanoate can also act as substrates [6].
Cofactor
Mg2+
History
Reactions
(1) Atp + cholate + CoA = amp + diphosphate + choloyl-CoA. (2) Atp + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26- oate + CoA = Amp + diphosphate + (25R)-3-alpha,7-alpha,12-alpha- trihydroxy-5-beta-cholestanoyl-CoA.
