This protein catalyses the successive addition of two glutamate residues to cofactor F420?by two distinct and independent reactions. In the reaction described here the enzyme attaches a glutamate via its ¦Á-amine group to F420-0. In the second reaction (EC?6.3.2.34, coenzyme F420-1¡ª¦Ã-L-glutamate ligase) it catalyses the addition of a second?L-glutamate residue to the ¦Ã-carboxyl of the first glutamate.
