This protein catalyses the successive addition of two glutamate residues to cofactor F420?by two distinct and independent reactions. In the first reaction (EC?6.3.2.31, coenzyme F420-0¡ªL-glutamate ligase) the enzyme attaches a glutamate via its ¦Á-amine group to F420-0. In the second reaction, which is described here, the enzyme catalyses the addition of a second?L-glutamate residue to the ¦Ã-carboxyl of the first glutamate.
