Cynomolgus Alkaline Phosphatase Protein (Placental type), Accession#: XP_045223825, CAS#: 3202-706-2483; ChemWhat Code: 1414969

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Cynomolgus Alkaline Phosphatase Protein (Placental type) is expressed from HEK293 with His tag at the C-terminal.It contains Ile21-Thr503.
Biotinylated

Specification

Target Alkaline Phosphatase
Synonyms
Gene/Protein ID (Accession) XP_045223825
Species Cynomolgus
Exact Sequence Ile21-Thr503
Molecular Weight The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1414969> 95% as determined by Tris-Bis PAGE
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