Cynomolgus IL-4 R alpha/CD124 Protein, Accession#: G7Q0S7, CAS#: 3202-706-2368; ChemWhat Code: 1414853
Overview
Product Description | Recombinant Cynomolgus IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Met26-Arg232. |
Biotinylated |
Specification
Target | IL-4 R alpha/CD124 |
Synonyms | |
Gene/Protein ID (Accession) | G7Q0S7 |
Species | Cynomolgus |
Exact Sequence | Met26-Arg232 |
Molecular Weight | The protein has a predicted MW of 24.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human IL-4, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 36.5ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-His |
Express System | HEK293 |
Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
Spectrums | |
Buy Reagent | |
ChemWhat 1414853 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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