The bifunctional protein?hldE?includes?D-glycero-¦Â-D-manno-heptose-7-phosphate kinase and?D-glycero-¦Â-D-manno-heptose 1-phosphate adenylyltransferase activity (cf. EC?2.7.1.167). The enzyme is involved in biosynthesis of ADP-L-glycero-¦Â-D-manno-heptose, which is utilized for assembly of the lipopolysaccharide inner core in Gram-negative bacteria.
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D-glycero-beta-D-manno-heptose 1-phosphate + Atp = ADP-D-glycero-beta-D- manno-heptose + diphosphate.