Human Alkaline Phosphatase Protein (Placental type), Accession#: P05187, CAS#: 3202-706-2395; ChemWhat Code: 1414887
Overview
| Product Description | Recombinant Human Alkaline Phosphatase Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ile23-Asp506. |
| Biotinylated |
Specification
| Target | Alkaline phosphatase |
| Synonyms | IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP |
| Gene/Protein ID (Accession) | P05187 |
| Species | Human |
| Exact Sequence | Ile23-Asp506 |
| Molecular Weight | The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414887 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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