Human APOH Protein, Accession#: P02749, CAS#: 3202-706-2507; ChemWhat Code: 1414979
Overview
| Product Description | Recombinant Human APOH Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gly20-Cys345. |
| Biotinylated |
Specification
| Target | APOH |
| Synonyms | Beta-2-glycoprotein 1; APC inhibitor; Apolipoprotein H; Apo-H; B2GP1; B2G1; APOH; B2GPI; beta(2)GPI; Beta-2-glycoprotein I; BG |
| Gene/Protein ID (Accession) | P02749 |
| Species | Human |
| Exact Sequence | Gly20-Cys345 |
| Molecular Weight | The protein has a predicted MW of 37.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human APOH, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.97ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414979 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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