Human CD10/MME Protein, Accession#: P08473, CAS#: 3202-706-2330; ChemWhat Code: 1414819
Overview
| Product Description | Recombinant Human CD10/MME Protein is expressed from HEK293 with His tag at the N-terminal.It contains Tyr52-Trp750. |
| Biotinylated |
Specification
| Target | CD10/MME |
| Synonyms | Neprilysin; Atriopeptidase; Enkephalinase; NEP; Neutral; SFE; CD10; MME; CALLA; DKFZp686O16152; MGC126681; MGC126707 |
| Gene/Protein ID (Accession) | P08473 |
| Species | Human |
| Exact Sequence | Tyr52-Trp750 |
| Molecular Weight | The protein has a predicted MW of 80.9 kDa. Due to glycosylation, the protein migrates to 81-110 kDa based on Tris-Bis PAGE result. |
| Activity | |
| Product Tag | N-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM MES, 100mM NaCl, 1mM ZnCl2, 10% Glycerol (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414819 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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