Human FGF basic (154aa) Protein, Accession#: P09038, CAS#: 3202-706-2099; ChemWhat Code: 1414599
Overview
| Product Description | Recombinant Human FGF basic (154aa) Protein is expressed from E.coli without tag.It contains Ala135-Ser288. |
| Biotinylated |
Specification
| Target | FGF basic (154aa) |
| Synonyms | |
| Gene/Protein ID (Accession) | P09038 |
| Species | Human |
| Exact Sequence | Ala135-Ser288 |
| Molecular Weight | The protein has a predicted MW of 17.12 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Human FGF basic (154aa) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | No Tag |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414599 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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