Human HPN Protein, Accession#: P05981, CAS#: 3202-706-2092; ChemWhat Code: 1414591

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human HPN Protein is expressed from HEK293 with His tag at the C-terminal.It contains Arg45-Leu417.
Biotinylated

Specification

Target HPN
Synonyms Hpxn; FLJ56652; Hemopexin; HPX; HX; Hepsin; HPN;TMPRSS1
Gene/Protein ID (Accession) P05981
Species Human
Exact Sequence Arg45-Leu417
Molecular Weight The protein has a predicted MW of 42 kDa. Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human HPN, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 20.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

Buy Reagent

ChemWhat 1414591> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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