Human KRAS G12D (HLA-C 03:04) Tetramer Protein, Accession#: QAV56463, CAS#: 3202-706-2359; ChemWhat Code: 1414849

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human KRAS G12D (HLA-C 03:04) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-C 03:04), Ile21-Met119 (B2M) and GADGVGKSAL peptide.
Biotinylated

Specification

Target KRAS G12D (HLA-C 03:04) Tetramer
Synonyms
Gene/Protein ID (Accession) QAV56463
Species Human
Exact Sequence Gly25-Thr305 (HLA-C 03:04), Ile21-Met119 (B2M) and GADGVGKSAL peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity
Product Tag C-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1414849> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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