Human L1CAM Protein, Accession#: P32004, CAS#: 3202-706-2085; ChemWhat Code: 1414579
Overview
| Product Description | Recombinant Human L1CAM Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ile20-Glu1120. |
| Biotinylated |
Specification
| Target | L1CAM |
| Synonyms | N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5; CALL; CHL1; CHL-1; FLJ44930; L1CAM-2; MGC132578; HSAS; HSAS1; MASA; S10; SPG1 |
| Gene/Protein ID (Accession) | P32004 |
| Species | Human |
| Exact Sequence | Ile20-Glu1120 |
| Molecular Weight | The protein has a predicted MW of 124.6 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human L1CAM, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414579 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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