Human MMP-8 Protein, Accession#: P22894, CAS#: 3202-706-1885; ChemWhat Code: 1414383
Overview
| Product Description | Recombinant Human MMP-8 Protein is expressed from HEK293 with His tag at the C-terminal. The protein needs to be activated by APMA to have hydrolytic activity.It contains Phe21-Gly467. |
| Biotinylated |
Specification
| Target | MMP-8 |
| Synonyms | MMP-8; PMNL-CL; CLG1; MMP8; Collagenase 2; PMNL-CL; HNC |
| Gene/Protein ID (Accession) | P22894 |
| Species | Human |
| Exact Sequence | Phe21-Gly467 |
| Molecular Weight | The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 3.5ug/ml determined by ELISA (QC Test). The affinity constant of 1.14 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM Tris,10mM CaCl2, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414383 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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