Human PRNP Protein, Accession#: P04156, CAS#: 3202-706-1782; ChemWhat Code: 1414275
Overview
| Product Description | Recombinant Human PRNP Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Lys23-Ser230. |
| Biotinylated |
Specification
| Target | PRNP |
| Synonyms | Major prion protein；PrP；ALTPRP；PRIP；PRP；PRNP |
| Gene/Protein ID (Accession) | P04156 |
| Species | Human |
| Exact Sequence | Lys23-Ser230 |
| Molecular Weight | The protein has a predicted MW of 49.4 kDa. Due to glycosylation, the protein migrates to 60-67 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human PRNP, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414275 | > 95% as determined by Tris-Bis PAGE |
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