Human PVRIG Protein, Accession#: Q6DKI7, CAS#: 3202-706-2060; ChemWhat Code: 1414557
Overview
| Product Description | Recombinant Human PVRIG Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminal.It contains Thr41-Asp171. |
| Biotinylated |
Specification
| Target | PVRIG |
| Synonyms | C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1 |
| Gene/Protein ID (Accession) | Q6DKI7 |
| Species | Human |
| Exact Sequence | Thr41-Asp171 |
| Molecular Weight | The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.22ug/ml determined by ELISA (QC Test). The affinity constant of 0.349 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-mFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414557 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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