Human R spondin 3/RSPO3 Protein, Accession#: Q9BXY4, CAS#: 3202-706-1938; ChemWhat Code: 1414433

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gln22-Val146.
Biotinylated

Specification

Target R spondin 3/RSPO3
Synonyms
Gene/Protein ID (Accession) Q9BXY4
Species Human
Exact Sequence Gln22-Val146
Molecular Weight The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human R-Spondin 3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22um filtered solution in PBS (pH 7.4).
Reconstitution
Stability and Storage Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

Buy Reagent

ChemWhat 1414433> 95% as determined by Tris-Bis PAGE
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