Human TGF-alpha Protein, Accession#: P01135, CAS#: 3202-706-2142; ChemWhat Code: 1414639
Overview
| Product Description | Recombinant Human TGF-alpha Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Val40-Ala89. |
| Biotinylated |
Specification
| Target | TGF-alpha |
| Synonyms | ETGF; TGF-alpha; TGF type 1; TGFA |
| Gene/Protein ID (Accession) | P01135 |
| Species | Human |
| Exact Sequence | Val40-Ala89 |
| Molecular Weight | The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414639 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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