Human TNFRSF11B Protein, Accession#: O00300, CAS#: 3202-706-1888; ChemWhat Code: 1414389
Overview
| Product Description | Recombinant Human TNFRSF11B Protein is expressed from HEK293 with His tag at the C-terminal.It contains Glu22-Leu401. |
| Biotinylated |
Specification
| Target | TNFRSF11B |
| Synonyms | |
| Gene/Protein ID (Accession) | O00300 |
| Species | Human |
| Exact Sequence | Glu22-Leu401 |
| Molecular Weight | The protein has a predicted MW of 44.71 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA. The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414389 | > 95% as determined by Tris-Bis PAGE |
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