CNAG_02565, HacA, HACN, Homoaconitase, LYS4, methanogen HACN, MJ1003, MJ1271, More
EC Number
4.2.1.114
CAS Registry Number
Comments
This enzyme catalyses several reactions in the pathway of coenzyme-B biosynthesis in methanogenic?Archaea. Requires a [4Fe-4S] cluster for activity. In contrast to EC?4.2.1.36, homoaconitate hydratase, this enzyme can catalyse both the dehydration of (R)-homocitrate to form?cis-homoaconitate and the subsequent hydration reaction that forms homoisocitrate. In addition to?cis-homoaconitate, the enzyme can also catalyse the hydration of the physiological substrates dihomocitrate and trihomocitrate as well as the non-physiological substrate tetrahomocitrate.?cis-Aconitate and?threo-DL-isocitrate cannot act as substrates, and (S)-homocitrate and trans-homoaconitate act as inhibitors of the enzyme.
