Requires Fe(III). The enzyme from the bacterium?Streptoalloteichus tenebrarius?catalyses the activation of carbamoyl phosphate to?O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin.
Cofactor
Fe3+
History
Reactions
(1) Tobramycin + carbamoyl phosphate + Atp + H(2)O = nebramycin 5′ + Amp + diphosphate + phosphate. (2) Kanamycin A + carbamoyl phosphate + atp + H(2)O = 6”-O- carbamoylkanamycin A + amp + diphosphate + phosphate.