The enzyme from the bacterium?Escherichia coli?is bifunctional and catalyses both the phosphorylation and dephosphorylation of EC?2.7.9.2, pyruvate, water dikinase.?cf. EC?2.7.4.28, ([pyruvate, water dikinase] phosphate) phosphotransferase [1]. The enzyme is specific for a reaction intermediate form of EC?2.7.9.2, where it phosphorylates an active site histidine [1]. It has no activity toward EC?2.7.9.1?pyruvate, phosphate dikinase (cf. EC?2.7.11.32, [pyruvate, phosphate dikinase] kinase).
Cofactor
History
Reactions
Adp + [pyruvate, water dikinase] = amp + [pyruvate, water dikinase] phosphate.