Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rHuTWEAK Receptor as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Gaudineau B, Fougere M, Guaddachi F, et al. 2012. J Cell Sci, 125: 4475-86.2. Abend JR, Uldrick T, Ziegelbauer JM. 2010. J Virol, 84: 12139-51.3. Kang S, Yang C, Luo R. 2008. Biochem Biophys Res Commun, 372: 629-33.4. Wiley SRandWinkles JA. 2003. Cytokine Growth Factor Rev, 14: 241-9.
