SUMO-protein Ligase, Ubiquitin Carrier Protein 9, Ubiquitin Carrier Protein I, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, p18
Accession Number
GeneID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids (a.a.) of human UBE2I/UBC9 and 8 a.a. vector sequence including 6 × His tag at N-terminus.
Biological Activity
No data available.
Appearance
Sterile Colorless liquid.
Formulation
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
Endotoxin
Less than 1 EU/ug of rHuUBC9, His as determined by LAL method.
Reconstitution
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
References
1. Yukita A, Hosoya A, Ito Y, et al. 2012. Bone, 50: 1092-9.2. Duan X, Trent JO, Ye H. 2009. Anticancer Agents Med Chem, 9: 51-4.3. Niedenthal R. 2009. Methods Mol Biol, 497: 63-79.4. Shang Q, Xu C, Zhang J, et al. 2009. Proteins, 76: 266-9.