L-UBC, UbcH7, Ubiquitin Carrier Protein L3, Ubiquitin-protein Ligase L3
Accession Number
GeneID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids (a.a.) of human UBE2L3/UBCH7 and 8 a.a. vector sequence including 6 × His tag at N-terminus.
Biological Activity
No data available.
Appearance
Sterile Colorless liquid.
Formulation
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
Endotoxin
Less than 1 EU/ug of rHuUBE2L3, His as determined by LAL method.
Reconstitution
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
References
1. Serniwka SAandShaw GS. 2008. Biomol NMR Assign, 2: 21-3.2. Wenzel DM, Lissounov A, Brzovic PS, et al. 2011. Nature, 474: 105-8.3. Whitcomb EAandTaylor A. 2009. Cell Div, 4: 17.4. Moynihan TP, Ardley HC, Nuber U, et al. 1999. J Biol Chem, 274: 30963-8.
