Recombinant Murine Breast and Kindey-expressed Chemokine/CXCL14 (rMuBRAK/CXCL14, Q9WUQ5, 57266) CAS#: 221-14-1816; ChemWhat Code: 1404598

Official Full Name Recombinant Murine Breast and Kindey-expressed Chemokine/CXCL14 (rMuBRAK/CXCL14)
Squence Squence of Recombinant Murine Breast and Kindey-expressed Chemokine/CXCL14
Amino Acid Sequence SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE
Synonyms B-cell and Monocyte-activating Chemokine, Chemokine BRAK, Kidney-expressed Chemokine CXC, MIP-2G, Small-inducible Cytokine B14
Accession Number Q9WUQ5
GeneID 57266
Summary
Source Escherichia coli.
Molecular Weight Approximately 9.4 kDa, a single non-glycosylated polypeptide chain containing 77amino acids.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 400 mM NaCl, pH 7.4, 5 % trehalose.
Endotoxin Less than 1 EU/ug of rMuBRAK/CXCL14 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Hromas R, Broxmeyer HE, Kim C, et al. 1999. Biochem Biophys Res Commun. 255:703-6.2. Starnes T, Rasila KK, Robertson MJ, et al. 2006. Exp Hematol. 34:1101-5.3. Shurin GV, Ferris RL, Tourkova IL, et al. 2005. J Immunol. 174:5490-8.4. Kurth I, Willimann K, Schaerli P, et al. 2001. J Exp Med. 194:855-61.5. Hara T, Tanegashima K. 2012. J Biochem. 151:469-76.6. Shellenberger TD, Wang M, Gujrati M, et al. 2004. Cancer Res. 64:8262-70.
SDS-PAGE SDS-PAGE of Recombinant Murine Breast and Kindey-expressed Chemokine/CXCL14
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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