Rhesus macaque Complement Factor D/CFD Protein, Accession#: H9EXC1, CAS#: 3202-706-2171; ChemWhat Code: 1414665

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Rhesus macaque Complement Factor D/CFD Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gln21-Ala253.
Biotinylated

Specification

Target Complement Factor D/CFD
Synonyms Adipsin; C3 convertase activator; Complement factor D; CFD; PFD; DF; ADN; FACTOR D; AMBP-1; EC 3.4.21; EC 3.4.21.46
Gene/Protein ID (Accession) H9EXC1
Species Rhesus macaque
Exact Sequence Gln21-Ala253
Molecular Weight The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Rhesus macaque Complement Factor D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1414665> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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