The enzyme, found in thermophilic bacteria such as?Thermus thermophilus?and archaea such as?Pyrococcus horikoshii, modifies the ribothymidine (5-methyluridine) residue at position 54 of tRNAs. Contains zinc.
Cofactor
Zn2+
History
Reactions
Atp + [TtuB sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + 5-methyluracil(54) in tRNA + H(2)O = Amp + diphosphate + 5-methyl-2- thiouracil(54) in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly.