In Escherichia coli TrmJ is the only methyltransferase responsible for the formation of 2′-O-methylcytidine32 in tRNA. No methylation of cytosine34 in tRNALeu(CAA). In vitro the enzyme 2′-O-methylates cytidine34 of tRNASer1 and uridine32 of tRNAGln2.
Cofactor
History
Reactions
(1) S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L- homocysteine + 2′-O-methylcytidine(32) in tRNA. (2) S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L- homocysteine + 2′-O-methyluridine(32) in tRNA.
