In contrast to the archaeal enzyme tRNA (guanine10-N2)-dimethyltransferase (EC 2.1.1.213), tRNA (guanine10-N2)-methyltransferase from yeast does not catalyse the methylation from N2-methylguanine10 to N2-dimethylguanine10 in tRNA.
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S-adenosyl-L-methionine + guanine(10) in tRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(10) in tRNA.