The enzyme from Aquifex aeolicus is similar to the TRM1 methyltransferases of archaea and eukarya (see EC 2.1.1.216, tRNA (guanine26-N2)-dimethyltransferase). However, it catalyses the double methylation of guanines at both positions 26 and 27 of tRNA.
Cofactor
History
Reactions
4 S-adenosyl-L-methionine + guanine(26)/guanine(27) in tRNA = 4 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26)/N(2)- dimethylguanine(27) in tRNA.