| Synonyms |
4,6-dehydratase/5-epimerase, CapE, Cj1293, FlaA1, HP0840, inverting 4,6-dehydratase, Mg534, More, NAD(P)+-dependent dehydratase/epimerase, PseB, UDP-alpha-D-GlcNAc modifying dehydratase, UDP-GlcNAc 5-inverting 4,6-dehydratase, UDP-GlcNAc C6 dehydratase, UDP-GlcNAc C6 dehydratase/C4 reductase, UDP-N-acetylglucosamine 5-inverting 4,6-dehydratase, WbpM |
| Comments |
Contains NADP+?as a cofactor. This is the first enzyme in the biosynthetic pathway of pseudaminic acid [3], a sialic-acid-like sugar that is unique to bacteria and is used by?Helicobacter pylori?to modify its flagellin. This enzyme plays a critical role in?H. pylori’s pathogenesis, being involved in the synthesis of both functional flagella and lipopolysaccharides [1,2]. It is completely inhibited by UDP-¦Á-D-galactose. The reaction results in the chirality of the C-5 atom being inverted. It is thought that Lys-133 acts sequentially as a catalytic acid, protonating the C-6 hydroxy group and as a catalytic base, abstracting the C-5 proton, resulting in the elimination of water. This enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes. |
